Nikon Instruments / Prodotti / Sistemi di Microscopia / Microscopi Confocali / A1R-A1 Confocal

Nikon A1R-A1 Confocal Microscope System

A1R-A1 Specifications

A1R

Input/output port:

Laser input port: 3 (FC x2, direct x1)
Signal output port: 4 (SMA x2, FC x1, VAAS x1)

Laser

 

Wavelength and power:

405LD: max. 38mW, Multi-Ar (457/488/514): max. 65mW, 488DPSS: max. 75mW, 561DPSS: max. 25mW, 543HeNe: max.
1mW, 638LD: max. 10mW
440LD: max. 15mW (available as option)

Modulation:

Method: AO (Acousto) device or drive current control
Control: power control for each wavelength, Return mask, ROI exposure control

Laser unit:

Standard: LU4 4-laser unit
Optional: C-LU3EX 3-laser unit EX

Standard fluorescence detector

 

Detector:

4 PMT

Filter cube:

6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths: 450/50, 482/35, 525/25, 595/50, 700/75, 540/30, 515/30, 585/65

Diascopic detector

 

Detector:

PMT

Scanning head

 

Scanning:

Scanning range: square inscribed in a ø18mm circle
Standard image acquisition
Scanner: non-resonant scanner x2
Pixel size: max. 4096 x 4096 pixels
Scanning speed: 4 fps (512 x 512 pixels)
Zoom: 1-1000x continuously variable
Scanning mode: X-Y, XY rotation, Free line, Line Z
High-speed image acquisition
Scanner: resonant scanner (X-axis, resonance frequency 7.8kHz),
non-resonant scanner (Y-axis)
Pixel size: max. 512 x 512 pixels
Scanning speed: 30 fps (512 x 512 pixels) to 230 fps
(512 x 64 pixels),
15,600 lines/sec (line speed)
Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
Scanning mode: X-Y, Line
Acquisition method: Standard image acquisition,
High-speed image acquisition, Simultaneous photo activation
and image acquisition

Dichroic mirror:

Low-angle incidence method
Position: 8
Standard filter: 405/488, 405/488/561, 405/488/561/638, 405/488/543/638, 457/514, BS20/80

Pinhole:

12-256µm variable (1st image plane)

Spectral detector

 

Number of channels:

32 channels

Spectral image acquisition speed:

4 fps (256 x 256 pixels), 1000 lps

Maximum wavelength and resolution:

80nm (2.5nm), 192nm (6nm), 320nm (10nm)
Wavelength range variable in 0.25nm steps

Unmixing:

High-speed unmixing, Precision unmixing

Z step:

0.025µm

Compatible microscopes:

ECLIPSE Ti-E inverted microscope,
ECLIPSE TE2000-E inverted microscope,
ECLIPSE 90i upright microscope,
ECLIPSE FN1 fixed stage microscope

Option:

Motorized XY stage, High-speed Z stage, VAAS

Software

 

Display/image generation:

2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing

Image format:

JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS

Application:

FRAP, FLIP, FRET, photo activation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization

Control computer

 

OS:

Microsoft Windows® XP 32bit SP (English version)

CPU:

Intel Xeon 5160 (3GHz/1333MHz/dual core) or higher

Memory:

4GB or more

Hard disk:

SAS (15,000rpm), 160GB or more x2, RAID 0 configuration

Data transfer:

Dedicated data transfer I/F

Monitor:

1600 x 1200 or higher resolution, 2 LCD monitor configuration recommended

Installation condition:

Temperature 5 - 35°C, humidity 65% (RH) or less (non-condensing)

Power Source

 

Controller:

Input voltage: 100–240VAC ±10% 50–60Hz
Current rating: 5A @100VAC
Overcurrent protection: main breaker 15A

LU-LR 4-laser power source rack:

Power source for Ar laser and control circuit: 100VAC,
15A/115VAC, 15A/230VAC, 7.5A, 50/60Hz (breaker 15A)
Power source for lasers except Ar laser: 100VAC, 3A/115VAC,
3A/230VAC, 1.5A, 50/60Hz (breaker 5A)

A1

Input/output port:

Laser input port: 3 (FC x2, direct x1)
Signal output port: 4 (SMA x2, FC x1, VAAS x1)

Laser

 

Wavelength and power:

405LD: max. 38mW, Multi-Ar (457/488/514): max. 65mW, 488DPSS: max. 75mW, 561DPSS: max. 25mW, 543HeNe: max.
1mW, 638LD: max. 10mW
440LD: max. 15mW (available as option)

Modulation:

Method: AO (Acousto) device or drive current control
Control: power control for each wavelength, Return mask, ROI exposure control

Laser unit:

Standard: LU4 4-laser unit
Optional: C-LU3EX 3-laser unit EX

Standard fluorescence detector

 

Detector:

4 PMT

Filter cube:

6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths: 450/50, 482/35, 525/25, 595/50, 700/75, 540/30, 515/30, 585/65

Diascopic detector

 

Detector:

PMT

Scanning head

 

Scanning:

Standard image acquisition
Scanner: non-resonant scanner x2
Pixel size: max. 4096 x 4096 pixels
Scanning speed: 4 fps (512 x 512 pixels)
Zoom: 1-1000x continuously variable
Scanning mode: X-Y, XY rotation, Free line, Line Z

Dichroic mirror:

Low-angle incidence method
Position: 8
Standard filter: 405/488, 405/488/561, 405/488/561/638, 405/488/543/638, 457/514, BS20/80

Pinhole:

12-256µm variable (1st image plane)

Spectral detector

 

Number of channels:

32 channels

Spectral image acquisition speed:

4 fps (256 x 256 pixels), 1000 lps

Maximum wavelength and resolution:

80nm (2.5nm), 192nm (6nm), 320nm (10nm)
Wavelength range variable in 0.25nm steps

Unmixing:

High-speed unmixing, Precision unmixing

Z step:

0.025µm

Compatible microscopes:

ECLIPSE Ti-E inverted microscope,
ECLIPSE TE2000-E inverted microscope,
ECLIPSE 90i upright microscope,
ECLIPSE FN1 fixed stage microscope

Option:

Motorized XY stage, High-speed Z stage, VAAS

Software

 

Display/image generation:

2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing

Image format:

JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS

Application:

FRAP, FLIP, FRET, photo activation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization

Control computer

 

OS:

Microsoft Windows® XP 32bit SP (English version)

CPU:

Intel Xeon 5160 (3GHz/1333MHz/dual core) or higher

Memory:

4GB or more

Hard disk:

SAS (15,000rpm), 160GB or more x2, RAID 0 configuration

Data transfer:

Dedicated data transfer I/F

Monitor:

1600 x 1200 or higher resolution, 2 LCD monitor configuration recommended

Installation condition:

Temperature 5 - 35°C, humidity 65% (RH) or less (non-condensing)

Power Source

 

Controller:

Input voltage: 100–240VAC ±10% 50–60Hz
Current rating: 5A @100VAC
Overcurrent protection: main breaker 15A

LU-LR 4-laser power source rack:

Power source for Ar laser and control circuit: 100VAC,
15A/115VAC, 15A/230VAC, 7.5A, 50/60Hz (breaker 15A)
Power source for lasers except Ar laser: 100VAC, 3A/115VAC,
3A/230VAC, 1.5A, 50/60Hz (breaker 5A)