Neurobiology

Confocal microscopy, optical sectioning and 3-D reconstruction are powerful techniques that allow cells of the nervous system be visualized deep into brain slices and other tissues. Similarly the use of specialized objectives designed for deep imaging such as water dipping and water immersion objectives improve results.

The development of brain tissue and neuronal outgrowth can be observed in detail in model organisms with translucent embryos using high-resolution light microscopy (confocal, fluorescence and DIC) and time-lapse imaging. Gene knock down, and enhanced gene expression techniques allow the role of specific neuronal growth factors and other signalling molecules in inducing neuronal outgrowths, synapse formation, and neuronal circuits to be studied. Cell migration can also be studied using fluorescence techniques involving powerful fluorophores, such as photactivatable GFPs, kaede and quantum dots.

The assembly of microtubules and their interaction with the cell membrane is involved in directing neurotransmitter vesicles to synapses. TIRF microscopy allows direct observations of microtubule function and vesicle movement within approximately 50-100 nm of the plasma membrane, while confocal microscopy can be used to monitor movement in the micron range. The pattern of calcium movement involved in modulating synaptic transmission can be observed with the help of fluorescent / luminescent calcium indicators and techniques such as FRAP and FLIP, while movement of calcium ions across the plasma membrane may be recorded using patch-clamp electrophysiology. The protein-protein interactions leading up to calcium release, in addition, can be monitored using techniques such as FRET and BRET.